Compared to AutoDelfia, additional control procedures were included which increase the reliability of the system. For the determination of recovery the respective kit calibrators were used. Table 4 Expected concentration, mean concentration and CV of kit controls for n -measurements over time. The t -test for independent samples was used to calculate the significance of the signal differences between blanks and analytes. After the final washing step these chelates are dissociated at pH 3. Cut-off for GSP, horizontal line ; cut-off for SC vertical line ; linear correlation ; logarithmic correlation.
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We were also not able to confirm the manufacturer's constraints that components from different kit-lots of the same assay are not interchangeable. For the determination of recovery the respective kit calibrators were used. Again the separate distribution of test-specific components coated microtiter plates, elution buffer , tracer and antiserum , rather than test-kits with a large amount of surplus elution buffer , tracer , and antiserum would save monetary resources and would additionally protect the environment. For the determination of the limit of detection LOD the statistical approach was used. For the statistical method either the lowest calibrator, if the respective analyte concentration was 0, was used i. For the TSH assay this is explained by the fact that even plain white filter paper, measured with the routine GSP-kit using a linear curve fit, results in a finite TSH-concentration of 0. The determination of the limit of quantitation LOQ is not so straightforward.